RT-SuperES is an EU-funded project that aims to bring a novel, affordable machine learning-based technology to market. It enables adaptive live and fixed-cell high-content imaging with unprecedented spatial and temporal resolution, revolutionizing nanoscale observations of cellular behavior. This groundbreaking system, a first-of-its-kind real-time super-resolution microscope, meets existing needs and creates a new premium market. It provides real-time features combined with advanced SR technology, advancing the field beyond the state-of-the-art and positioning the European industry uniquely for new possibilities, markets, and research opportunities.